邮箱：2216883001@bjmu.edu.cn

地址：北大医疗产业园12号一层精准医疗多组学研究中心

    

个人简介:  

        

        田文敏博士长期致力于质谱蛋白质组学新技术和新方法的开发，包括微量蛋白质组学、蛋白质糖修饰组学和临床样本的深度定量等前沿技术的        开发，并利用临床队列蛋白质组学方法深入解析疾病发生机制、开发疾病预警生物标志物。研究成果发表在Cell Research、Nature Communications、Journal of Medical Virology、Communications Biology 、Journal of Proteome Research等杂志。主持和参与国家自然科学青年项目和原创探索项目。现任北京神经科学学会自由基医学专业委员会委员。

主要研究方向:  

        利用基于质谱的蛋白质组学手段解析了多种疾病的发生机制，包括新型冠状病毒爆发初期，我们从患者尿液蛋白质组中发现的病毒感染后机体出现先抑制后激活的免疫应答现象，为临床用药提供了理论依据。我们还建立了蛋白质糖基化修饰鉴定和数据分析的全套方法，成功的解析了新型冠状病毒刺突蛋白糖基化修饰的全谱，发现了O-糖基化修饰追随N-糖基化修饰发生的现象。

        血液作为最常见和最易获得的体液样本，蛋白质鉴定深度低一直是领域内亟待解决的问题。我们利用分级策略开发了基于血液样本的深度蛋白质组学方法。在血清/血浆样本中定量了5000多个蛋白质。该方法具有高稳定性以及操作简单等特点，适用于大规模临床队列研究，并成功应用于多种疾病的发生机制解析和生物标志物开发的研究中。

        

基于质谱的临床样本深度蛋白质组技术解析疾病发生机制和开发生物标志物

代表性科研项目：

        1. 利用质谱手段发现蛋白质糖基化O follow N 的规律

        2. 利用分级手段实现单个血清样本定量蛋白质组5000+以上蛋白质的鉴定数

10篇代表性论文：

        1. Huang S*, Tian W*, Tian J, Tang H, Qin M, Zhao B, Wang J, Chen Y, Xu H. Deep Saliva Proteomics Elucidating the Pathogenesis of Early Childhood Caries and Identifying Biomarkers for Early Prediction. J Proteome Res. 2025 Feb 7;24(2):750-761. (IF:3.8)

        2. Jian F, Wang S, Tian W, Chen Y, Wang S, Li Y, Ma C, Rong Y. Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17. Nat Commun. 2025 Jan 9;16(1):543. (IF:14.7)

        3. Tian W*, Han Z*, Shi D*, Wang H, Tang H, Wu Z, Zhang Y, Cui L, Shen N, Zheng J, Chen Y. Indeterminate Result of Interferon-γ Release Assay-A Risk Factor of Mortality for COVID-19 and Non-COVID-19 Respiratory Infections. J Med Virol. 2024 Nov;96(11):e70058. (IF:6.8)

        4. Wang L*, Tian W*, Wang S*, Liu Y, Wang H, Xiao J, Yu Z, Xie L, Chen Y. Serum proteomics identifies biomarkers for predicting non-survivors in elderly COVID-19 patients. J Proteomics. 2024 Nov 14;311:105356. (IF:2.8)

        5. Wenmin Tian*, DongXue Shi*, Yinmei Zhang*, Hongli Wang, Haohao Tang, Zhongyu Han, Catherine CL Wong, Liyan Cui, Jiajia Zheng, Yang Chen. Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of large and small circulating extracellular vesicle enriched fraction. Communications Biology, 2024 Jan 15;7(1):99. (IF:5.9)

        6. Yuan Zhao, Chenxuan Zhou, Wenmin Tian, Yang Chen, Chongchong Zhao, Yan Li, Shixuan Wang, Yueguang Rong. A specific module of ESCRT regulates STING activity termination by controlling STING degradation. Sci Bull (Beijing), 2024 Jan 5:S2095-9273(24)00010-0. (IF:18.9)

        7. Yinmei Zhang*, Shangjia Jin*, Wenmin Tian*, Dongxue Shi, Yang Chen, Liyan Cui, Jiajia Zheng. Proteomic of serum samples for the exploration of the pathological mechanism of Obstetric Antiphospholipid Syndrome. Journal of Proteome Research, J Proteome Res, 2024 Jan 5;23(1):289-300.. (IF: 5.37)

        8. Wenmin Tian*, Delin Li*, Nan Zhang*, Guijie Bai*, Kai Yuan*, Haixia Xiao, Feng Gao, Yang Chen, Catherine CL Wong, George Fu Gao. O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an “O-Follow-N" rule. Cell Research, 2021 Oct 31(10):1123-1125. (IF: 25.617)

        9. Wenmin Tian*, Nan Zhang*, Ronghua Jin*, Yingmei Feng*, SiyuanWang*, Shuaixin Gao, Ruqin Gao, Guizhen Wu, Di Tian, Wenjie Tan, Yang Chen, George Fu Gao, Catherine C. L. Wong. Immune suppression in the early stage of COVID-19 disease. Nat Commun, 2020 Nov 17;11(1):5859. (IF: 14.919)

        10. Cuitong He, Jiangtao Guo, Wenmin Tian, and Catherine C. L. Wong. Proteogenomics Integrating Novel Junction Peptide Identification Strategy Discovers Three Novel Protein Isoforms of Human NHSL1 and EEF1B2. J Proteome Res. 2021 Aug 21. (IF: 4.466)